Journal: Frontiers in Immunology

Article Title: Artificial neural network-based analysis of ferroptosis-associated molecular subtypes and immunological profiles in abdominal aortic aneurysm

doi: 10.3389/fimmu.2026.1721069

Figure Lengend Snippet: Validation of the expression of the three candidate biomarkers in clinical specimens of AAA with immunofluorescence (IF) staining. (A B) Relative expressions of Oncostatin M (OSM) in AAA tissues and aneurysm neck controls, as determined by IF techniques. (C, D) Relative expressions of Heme oxygenase-1 (HMOX-1) in AAA tissues and aneurysm neck controls, as determined by IF techniques. (E, F) Relative expressions of interleukin-6 (IL-6) in AAA tissues and aneurysm neck controls, as determined by IF techniques. Mean ± SEM, n = 6. ****P <0.0001.

Article Snippet: After deparaffinization, rehydration, and antigen retrieval, sections were incubated overnight at 4°C with primary antibodies against human Oncostatin M (OSM; ABclonal, A8705), Heme oxygenase−1 (HMOX-1; Proteintech, 10701−1−AP), or interleukin−6 (IL−6; Boster, BA4339), followed by appropriate fluorescent secondary antibodies and DAPI counterstaining.

Techniques: Biomarker Discovery, Expressing, Immunofluorescence, Staining